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pet15b prokaryotic expression vector  (Millipore)


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    Structured Review

    Millipore pet15b prokaryotic expression vector
    Pet15b Prokaryotic Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet15b+expression+vector/pet28a/pmc10954247-724-9-13
    Average 90 stars, based on 1 article reviews
    pet15b prokaryotic expression vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis
    Article Snippet: .. The PCR products were cloned into pET15b expression vector (Novagen) between NdeI and BamHI restriction sites, resulting in the introduction of a hexa-His tag at the N-terminus of the expressed proteins. ..

    Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy.
    Article Snippet: .. The primer pairs used for PCR-amplification of the PMT coding gene fragments for directional cloning in the pET15b expression vector (Novagen) in-frame with the N-terminal hexahistidine tag (His tag) are listed in Table S7. .. The recombinant expression vectors were sequenced to confirm identity of the PMT insert, amplified in the K-12 strain of E. coli cells (NEB Turbo; New England Biolabs) and transformed into protein expression BL21-CodonPlus (DE3)-RIL E. coli (Agilent Technologies).

    Clone Assay:

    Article Title: Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis
    Article Snippet: .. The PCR products were cloned into pET15b expression vector (Novagen) between NdeI and BamHI restriction sites, resulting in the introduction of a hexa-His tag at the N-terminus of the expressed proteins. ..

    Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
    Article Snippet: .. The fragments were digested with appropriate restriction enzymes and cloned in the pET15b expression vector (EMD Millipore). ..

    Article Title: Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.
    Article Snippet: .. DNA encoding E. coli MAP (UniProt ID P0AE18) was amplified from E. coli TG1 genomic DNA and cloned into a pET15b expression vector (Novagen). ..

    Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
    Article Snippet: .. The fragments were digested with appropriate restriction enzymes and cloned in the pET15b expression vector (EMD Millipore). ..

    Expressing:

    Article Title: Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis
    Article Snippet: .. The PCR products were cloned into pET15b expression vector (Novagen) between NdeI and BamHI restriction sites, resulting in the introduction of a hexa-His tag at the N-terminus of the expressed proteins. ..

    Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy.
    Article Snippet: .. The primer pairs used for PCR-amplification of the PMT coding gene fragments for directional cloning in the pET15b expression vector (Novagen) in-frame with the N-terminal hexahistidine tag (His tag) are listed in Table S7. .. The recombinant expression vectors were sequenced to confirm identity of the PMT insert, amplified in the K-12 strain of E. coli cells (NEB Turbo; New England Biolabs) and transformed into protein expression BL21-CodonPlus (DE3)-RIL E. coli (Agilent Technologies).

    Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
    Article Snippet: .. The fragments were digested with appropriate restriction enzymes and cloned in the pET15b expression vector (EMD Millipore). ..

    Article Title: Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies.
    Article Snippet: DNA sequences were codon optimized for expression in E. coli, and synthesized by BioBasic Inc. (Markham ON Canada). .. The synthetic DNA was inserted into a pET15b expression vector (Novagen) at the NcoI and BamHI sites. ..

    Article Title: Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.
    Article Snippet: .. DNA encoding E. coli MAP (UniProt ID P0AE18) was amplified from E. coli TG1 genomic DNA and cloned into a pET15b expression vector (Novagen). ..

    Article Title: Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii ( Pichia pastoris ) and Escherichia coli for NMR studies
    Article Snippet: DNA sequences were codon optimized for expression in E. coli , and synthesized by BioBasic Inc. (Markham ON Canada). .. The synthetic DNA was inserted into a pET15b expression vector (Novagen) at the NcoI and Bam HI sites. ..

    Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
    Article Snippet: .. The fragments were digested with appropriate restriction enzymes and cloned in the pET15b expression vector (EMD Millipore). ..

    Plasmid Preparation:

    Article Title: Mitochondrial ferredoxin-like is essential for forming complex I-containing supercomplexes in Arabidopsis
    Article Snippet: .. The PCR products were cloned into pET15b expression vector (Novagen) between NdeI and BamHI restriction sites, resulting in the introduction of a hexa-His tag at the N-terminus of the expressed proteins. ..

    Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy.
    Article Snippet: .. The primer pairs used for PCR-amplification of the PMT coding gene fragments for directional cloning in the pET15b expression vector (Novagen) in-frame with the N-terminal hexahistidine tag (His tag) are listed in Table S7. .. The recombinant expression vectors were sequenced to confirm identity of the PMT insert, amplified in the K-12 strain of E. coli cells (NEB Turbo; New England Biolabs) and transformed into protein expression BL21-CodonPlus (DE3)-RIL E. coli (Agilent Technologies).

    Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
    Article Snippet: .. The fragments were digested with appropriate restriction enzymes and cloned in the pET15b expression vector (EMD Millipore). ..

    Article Title: Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies.
    Article Snippet: DNA sequences were codon optimized for expression in E. coli, and synthesized by BioBasic Inc. (Markham ON Canada). .. The synthetic DNA was inserted into a pET15b expression vector (Novagen) at the NcoI and BamHI sites. ..

    Article Title: Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.
    Article Snippet: .. DNA encoding E. coli MAP (UniProt ID P0AE18) was amplified from E. coli TG1 genomic DNA and cloned into a pET15b expression vector (Novagen). ..

    Article Title: The CIS association of CD47 with integrin Mac-1 regulates macrophage responses by stabilizing the extended integrin conformation
    Article Snippet: .. The fragments were digested with appropriate restriction enzymes and cloned in the pET15b expression vector (EMD Millipore). ..

    Cloning:

    Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy.
    Article Snippet: .. The primer pairs used for PCR-amplification of the PMT coding gene fragments for directional cloning in the pET15b expression vector (Novagen) in-frame with the N-terminal hexahistidine tag (His tag) are listed in Table S7. .. The recombinant expression vectors were sequenced to confirm identity of the PMT insert, amplified in the K-12 strain of E. coli cells (NEB Turbo; New England Biolabs) and transformed into protein expression BL21-CodonPlus (DE3)-RIL E. coli (Agilent Technologies).

    Article Title: Broad-Spectrum Inhibitors for Conserved Unique Phosphoethanolamine Methyltransferases in Parasitic Nematodes Possess Anthelmintic Efficacy
    Article Snippet: .. The primer pairs used for PCR-amplification of the PMT coding gene fragments for directional cloning in the pET15b expression vector (Novagen) in-frame with the N-terminal hexahistidine tag (His tag) are listed in Table S7. .. The recombinant expression vectors were sequenced to confirm identity of the PMT insert, amplified in the K-12 strain of E. coli cells (NEB Turbo; New England Biolabs) and transformed into protein expression BL21-CodonPlus (DE3)-RIL E. coli (Agilent Technologies).

    Amplification:

    Article Title: Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.
    Article Snippet: .. DNA encoding E. coli MAP (UniProt ID P0AE18) was amplified from E. coli TG1 genomic DNA and cloned into a pET15b expression vector (Novagen). ..



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